ABSTRACT
Objective: To establish a preparation method for verbascoside and isoverbascoside with high purity from Pediculariskansuensis. Methods: With the contents of verbascoside and isoverbascoside as indexes, static and dynamic adsorption-desorption were used to select the best macroporous resin from 01A1, D101, HPD100, AB-8, XAD-6, DM130, DM-301, DM-201, YWD06B, and YWD06C. The purification technology parameters of total phenylethanoid glycosides (PhGs) from P. kansuensis were optimized by selected macroporous resin. Medium pressure column chromatography was further used to purify verbascoside and isoverbascoside. The chemical structures were identified on the basis of the spectral data. Results: D101 type resin showed the best purifying profile, its optimum technology conditions were as follows: Sample injection concentration was 20 mg/mL, sample flow rate was 3 BV/h, then washing off the impurity with 3 BV deionized water, and being eluted by 5 BV 50% ethanol with the desorption rate at 2 BV/h. The 50% ethanol fraction was evaporated and dried with cry desiccation to obtain the total PhGs. The contents of verbascoside and isoverbascoside in total PhGs were 42.29% and 28.51%, respectively. After purified by medium pressure column chromatography, the contents of prepared verbascoside and isoverbascoside reached 98.3% and 99.2%, respectively. Conclusion: This method can be used for the large-scale preparation of verbascoside and isoverbascoside with high purity, which is rapid, simple, and practicable with high efficiency.